Volume 1 , Issue 1 , May 2013 , Pages: 7 - 14
Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid
Lukianova N Yu., Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Naleskina L A., Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Bezdenezhnykh N O., Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Kunskaya L M., Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Demash D V., Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Yanish Yu V., Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Todor I N., Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Chekhun V F., Department of Mechanisms of Antitumor Therapy, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Received: May 14, 2013;       Published: Jun. 10, 2013
DOI: 10.11648/j.crj.20130101.12        View        Downloads  
Abstract
The peculiarities of changes of phenotype properties in MCF-7 human breast cancer cells with different sensitivity to cytostatic agents under the influence of nanoparticles of magnetic fluid were studied. It is defined that the biggest manifestations of magnetic fluid effect both by its essence and direction are observed in cells of resistant MCF-7/Dox line and parental MCF-7/S line in comparison with MCF-7/CP (cisplatin-resistant cell line). In particular, these are more apparent cytotoxic effects and ability to colony-formation, increase of adhesive properties, decrease of invasiveness and proliferative activity, changes of surface charge density and electrokinetic potential as well as lipid composition of cells. The defined differences between MCF-7 cells with different sensitivity to cytostatic agents according to phenotypic manifestations of correlations (magnetic fluid effect – reactive response) are evidence of the individual peculiarities of free radicals formation in cells and development of oxidative stress. The indicated phenomenon of selective reactive changes in cells by effect of magnetic fluid is necessary to take into account in development of systems of directed transport of antitumor drugs.
Keywords
MCF-7 Cells, Nanoparticles of Magnetic Fluid, Drug Resistance, Cytotoxic Effects, Adhesion, Invasion, Proliferative Activity, Free Radical Processes, Oxidative Stress, Lipids
To cite this article
Lukianova N Yu., Naleskina L A., Bezdenezhnykh N O., Kunskaya L M., Demash D V., Yanish Yu V., Todor I N., Chekhun V F., Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid, Cancer Research Journal. Vol. 1, No. 1, 2013, pp. 7-14. doi: 10.11648/j.crj.20130101.12
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