Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan

Hala Abdalgader Khairalseed Abas; Hind Abdelaziz Elnasri; Mona Abdelrahman Mohmed Khaier

doi:doi:10.11648/j.crj.20210902.12

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Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier

Published in Cancer Research Journal (Volume 9, Issue 2)

Received: 31 May 2020 Accepted: 4 July 2020 Published: 8 May 2021

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Abstract

Background: The aim of this study was to investigate the possible risk factors and mutations in VIM gene among Sudanese’s breast cancer women in Khartoum State. Methods: This case-control study involved 45 patients with breast cancer and 45controls. It was conducted across three hospitals and two laboratories in Khartoum State. A structural questionnaire was used to obtain data regarding age, family history, menarche, marriage, menopause, pregnancy, nulliparous and parous women, breast-feeding, use of fertility or contraceptive drugs and grade of the disease. DNA from patient and control tissues was extracted using extraction kits. PCR was conducted to amplify VIM gene using specific primers. PCR products were sequenced in order to detect the mutation in VIM gene. Data was analyzed using Pearson’s Chi-square tests to identify risk factors associated with breast cancer. Results: The study showed that the main risk factors associated with breast cancer were family history with first degree relative, menarche, irregularity of menarche, reproductive factors such as pregnancy, breast-feeding and nulliparous. DNA sequencing revealed no mutations in VIM gene associated with breast cancer in Sudanese women in Khartoum State. Conclusion: The association of other risk factors such as menopausal status, age of menopausal, oral contraceptive birth control and fertility hormones needs more illumination and further work. Other genes associated with breast cancer can be investigated.

Published in	Cancer Research Journal (Volume 9, Issue 2)
DOI	10.11648/j.crj.20210902.12
Page(s)	92-97
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Breast Cancer, Risk Factors, Sudan, VIM Gene

References

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APA Style

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier. (2021). Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Research Journal, 9(2), 92-97. https://doi.org/10.11648/j.crj.20210902.12

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ACS Style

Hala Abdalgader Khairalseed Abas; Hind Abdelaziz Elnasri; Mona Abdelrahman Mohmed Khaier. Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Res. J. 2021, 9(2), 92-97. doi: 10.11648/j.crj.20210902.12

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AMA Style

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier. Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Res J. 2021;9(2):92-97. doi: 10.11648/j.crj.20210902.12

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@article{10.11648/j.crj.20210902.12,
  author = {Hala Abdalgader Khairalseed Abas and Hind Abdelaziz Elnasri and Mona Abdelrahman Mohmed Khaier},
  title = {Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan},
  journal = {Cancer Research Journal},
  volume = {9},
  number = {2},
  pages = {92-97},
  doi = {10.11648/j.crj.20210902.12},
  url = {https://doi.org/10.11648/j.crj.20210902.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20210902.12},
  abstract = {Background: The aim of this study was to investigate the possible risk factors and mutations in VIM gene among Sudanese’s breast cancer women in Khartoum State. Methods: This case-control study involved 45 patients with breast cancer and 45controls. It was conducted across three hospitals and two laboratories in Khartoum State. A structural questionnaire was used to obtain data regarding age, family history, menarche, marriage, menopause, pregnancy, nulliparous and parous women, breast-feeding, use of fertility or contraceptive drugs and grade of the disease. DNA from patient and control tissues was extracted using extraction kits. PCR was conducted to amplify VIM gene using specific primers. PCR products were sequenced in order to detect the mutation in VIM gene. Data was analyzed using Pearson’s Chi-square tests to identify risk factors associated with breast cancer. Results: The study showed that the main risk factors associated with breast cancer were family history with first degree relative, menarche, irregularity of menarche, reproductive factors such as pregnancy, breast-feeding and nulliparous. DNA sequencing revealed no mutations in VIM gene associated with breast cancer in Sudanese women in Khartoum State. Conclusion: The association of other risk factors such as menopausal status, age of menopausal, oral contraceptive birth control and fertility hormones needs more illumination and further work. Other genes associated with breast cancer can be investigated.},
 year = {2021}
}

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TY - JOUR
T1 - Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan
AU - Hala Abdalgader Khairalseed Abas
AU - Hind Abdelaziz Elnasri
AU - Mona Abdelrahman Mohmed Khaier
Y1 - 2021/05/08
PY - 2021
N1 - https://doi.org/10.11648/j.crj.20210902.12
DO - 10.11648/j.crj.20210902.12
T2 - Cancer Research Journal
JF - Cancer Research Journal
JO - Cancer Research Journal
SP - 92
EP - 97
PB - Science Publishing Group
SN - 2330-8214
UR - https://doi.org/10.11648/j.crj.20210902.12
AB - Background: The aim of this study was to investigate the possible risk factors and mutations in VIM gene among Sudanese’s breast cancer women in Khartoum State. Methods: This case-control study involved 45 patients with breast cancer and 45controls. It was conducted across three hospitals and two laboratories in Khartoum State. A structural questionnaire was used to obtain data regarding age, family history, menarche, marriage, menopause, pregnancy, nulliparous and parous women, breast-feeding, use of fertility or contraceptive drugs and grade of the disease. DNA from patient and control tissues was extracted using extraction kits. PCR was conducted to amplify VIM gene using specific primers. PCR products were sequenced in order to detect the mutation in VIM gene. Data was analyzed using Pearson’s Chi-square tests to identify risk factors associated with breast cancer. Results: The study showed that the main risk factors associated with breast cancer were family history with first degree relative, menarche, irregularity of menarche, reproductive factors such as pregnancy, breast-feeding and nulliparous. DNA sequencing revealed no mutations in VIM gene associated with breast cancer in Sudanese women in Khartoum State. Conclusion: The association of other risk factors such as menopausal status, age of menopausal, oral contraceptive birth control and fertility hormones needs more illumination and further work. Other genes associated with breast cancer can be investigated.
VL - 9
IS - 2
ER -

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Author Information

Hala Abdalgader Khairalseed Abas

Center of Medical Research, Faculty of Medicine, Al-Neelain University, Khartoum, Sudan
Hind Abdelaziz Elnasri

Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan
Mona Abdelrahman Mohmed Khaier

Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan

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Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier. (2021). Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Research Journal, 9(2), 92-97. https://doi.org/10.11648/j.crj.20210902.12

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Hala Abdalgader Khairalseed Abas; Hind Abdelaziz Elnasri; Mona Abdelrahman Mohmed Khaier. Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Res. J. 2021, 9(2), 92-97. doi: 10.11648/j.crj.20210902.12

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AMA Style

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier. Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Res J. 2021;9(2):92-97. doi: 10.11648/j.crj.20210902.12

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@article{10.11648/j.crj.20210902.12,
  author = {Hala Abdalgader Khairalseed Abas and Hind Abdelaziz Elnasri and Mona Abdelrahman Mohmed Khaier},
  title = {Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan},
  journal = {Cancer Research Journal},
  volume = {9},
  number = {2},
  pages = {92-97},
  doi = {10.11648/j.crj.20210902.12},
  url = {https://doi.org/10.11648/j.crj.20210902.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20210902.12},
  abstract = {Background: The aim of this study was to investigate the possible risk factors and mutations in VIM gene among Sudanese’s breast cancer women in Khartoum State. Methods: This case-control study involved 45 patients with breast cancer and 45controls. It was conducted across three hospitals and two laboratories in Khartoum State. A structural questionnaire was used to obtain data regarding age, family history, menarche, marriage, menopause, pregnancy, nulliparous and parous women, breast-feeding, use of fertility or contraceptive drugs and grade of the disease. DNA from patient and control tissues was extracted using extraction kits. PCR was conducted to amplify VIM gene using specific primers. PCR products were sequenced in order to detect the mutation in VIM gene. Data was analyzed using Pearson’s Chi-square tests to identify risk factors associated with breast cancer. Results: The study showed that the main risk factors associated with breast cancer were family history with first degree relative, menarche, irregularity of menarche, reproductive factors such as pregnancy, breast-feeding and nulliparous. DNA sequencing revealed no mutations in VIM gene associated with breast cancer in Sudanese women in Khartoum State. Conclusion: The association of other risk factors such as menopausal status, age of menopausal, oral contraceptive birth control and fertility hormones needs more illumination and further work. Other genes associated with breast cancer can be investigated.},
 year = {2021}
}

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