Perfusion of Multiple Micronutrients Supplement of HCC-Invaded Human Liver Reduces Oxidative Stress and Proliferation of Cancer Cells
Torricelli Piera,
Ferorelli Pasquale,
Shevchenko Anna,
Antonelli Francesco,
Siciliano Alberto,
De Martino Angelo,
Beninati Simone
Issue:
Volume 4, Issue 5, September 2016
Pages:
69-72
Received:
4 August 2016
Accepted:
26 August 2016
Published:
21 September 2016
Abstract: Administration of the chemotherapeutic agent by organ perfusion is more effective than the systemic administration. The active drug is reached by the tumor, to a higher concentration, and normal tissues are spared from major damages. The perfusion of various areas of the body is a recent goal of the experimental and clinical trials. Fatal malignancies observed in humans occur mainly in the abdominal area of the body, The development of a clinically applicable technique for the perfusion of this area is desirable. This report covers the laboratory studies, carried out in the development of a liver perfusion technique for chemotherapy. The results obtained have allowed us to highlight that, the potential anticancer activity of Citozym, a micronutrients supplement, is likely the result of the inhibition of oxidative stress. This work is the preliminary evidence, of the possible mechanism by which Citozym exerts its antineoplastic activity.
Abstract: Administration of the chemotherapeutic agent by organ perfusion is more effective than the systemic administration. The active drug is reached by the tumor, to a higher concentration, and normal tissues are spared from major damages. The perfusion of various areas of the body is a recent goal of the experimental and clinical trials. Fatal malignanc...
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Copper Enhances Antitumor Activity of Water Soluble Garlic Extract Components on a Human Hepatoma Cell Line
Angelo De Martino,
Hanan Mahmoud Abu-Zeid,
Piera Torricelli,
Anna Shevchenko,
Alberto Siciliano,
Simone Beninati
Issue:
Volume 4, Issue 5, September 2016
Pages:
73-83
Received:
3 August 2016
Accepted:
31 August 2016
Published:
21 September 2016
Abstract: Water garlic extract combined with Copper (GE-Cu) shows specific anti-proliferative and antitumoral activity in human hepatocarcinoma (HepG2) cancer cell line, while it is not active on differentiated or primary isolated cells, normal human dermal fibroblasts (HDF) and human epidermal keratinocytes (HEK). Since other transition metals do not affect cell viability, the observed antineoplastic property, was found specific for copper (Cu), and atomic absorption analyses demonstrate an accumulation of this metal inside the cell nuclei. GE-Cu treatment of HepG2a cancer cells, induces cell differentiation, growth arrest and programmed cell death. Furthermore, GE-Cu produces a continuous and prolonged flux of oxidative radicals, which down-regulates nuclear antioxidant defenses and repair systems, thus generating direct double-strand breaks to DNA. Indeed, it was observed that in GE-Cu-treated HepG2 cancer cells, histone deacetylase (HDAC) is inhibited, and histone H2AX is early phospho-activated. DNA double strand break downstream, likely responsible for the observed cell death in HepG2 cell, was evidenced by an early over expression of p53 and p21. JNK/c-Jun and p-38 phosphorylative cascade are activated as response to oxidative stress also. The resulting apoptosis appears to be caspases-independent because, under our experimental conditions, nuclear translocation of the apoptosis inducing factor (AIF) is operative. Our data suggest that GE-Cu possess an active specific antitumor capability, which could provide valuable new tools in cancer prevention and in supporting traditional chemotherapy.
Abstract: Water garlic extract combined with Copper (GE-Cu) shows specific anti-proliferative and antitumoral activity in human hepatocarcinoma (HepG2) cancer cell line, while it is not active on differentiated or primary isolated cells, normal human dermal fibroblasts (HDF) and human epidermal keratinocytes (HEK). Since other transition metals do not affect...
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