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Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer
Hailong Zhu,
Xuyou Zhu,
Xiaoli Li,
Ran Cui
Issue:
Volume 11, Issue 3, September 2023
Pages:
86-90
Received:
24 March 2023
Accepted:
12 April 2023
Published:
6 July 2023
Abstract: Introduction: MicroRNA-218 (miR-218) acts as a tumor suppressor in various types of cancer. However, the association between miR-218-5p, the mature body of miR-218, and colorectal cancer (CRC) metastasis remains unclear. Objective: This study aimed to investigate the effect of miR-218-5p on CRC metastasis in vivo and the underlying molecular mechanism. Methods: Quantitative real-time PCR with reverse transcription (RT-qPCR) was employed to determine the expression level of miR-218-5p that trying to investigate if there is a link to clinicopathological characteristics. HCT116 cells were transfected with lentivirus for stably expressing miR-218-5p which was validated, as well as its target gene CDH2, by RT-qPCR and Western blotting. To generate xenograft models, BALB/c nude mice were treated with transfected cells for stably overexpressing miR-218-5p. Finally, the lung metastasis of CRC was observed. Results: The miR-218-5p expression was significantly downregulated in CRC tissues, playing the role of indicator for lymph node metastasis, advanced clinical stage, and poor prognosis. In addition, the expression level of CDH2 was negatively related to miR-218-5p in lentiviral transfected HCT116 cells. At the same time, overexpressed miR-218-5p inhibited CRC cell xenografts metastasis. Conclusion: Data indicates that miR-218-5p suppresses the metastasis of CRC cells, in part by inhibiting CDH2.
Abstract: Introduction: MicroRNA-218 (miR-218) acts as a tumor suppressor in various types of cancer. However, the association between miR-218-5p, the mature body of miR-218, and colorectal cancer (CRC) metastasis remains unclear. Objective: This study aimed to investigate the effect of miR-218-5p on CRC metastasis in vivo and the underlying molecular mechan...
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Gastric Cancers: Surgical Management and Prognostic Factors in the General Surgery Department of the National Ignace Deen Hospital, Conakry (Guinea)
Conde Ansoumane,
Fofana Naby,
Soumaoro Labile Togba,
Kondano Saa Yawo,
Fofana Houssein,
Toure Aboubacar
Issue:
Volume 11, Issue 3, September 2023
Pages:
91-93
Received:
27 May 2023
Accepted:
13 June 2023
Published:
17 July 2023
Abstract: Introduction: gastric cancer remains one of the leading causes of cancer death worldwide. The aim of this work was to study the surgical management and to determine the prognostic factors of gastric cancer in the general surgery department of the Ignace Deen national hospital, Conakry (Guinea). Patients and methods: This was a retrospective descriptive study covering a period of 10 years (January 2012 - December 2021) on the consecutive records of patients operated on for gastric cancer in the general surgery department of the Ignace Deen national hospital, Conakry. The variables studied concerned sociodemographic, clinical, therapeutic, histological and prognostic aspects. Results: During the 10 years, we collected 57 gastric cancer cases representing 40.7% of all digestive cancers. The average age of the patients was 57 ± 27.3 years (extremes: 19 and 90 years) with a male predominance (64.9%). In the majority of cases, the cancer had a budding aspect (57.9%) and was located in the antro-pyloric region (71.9%). In the management, curative gastrectomy was possible in 35.1% of cases against 61.4% of palliative surgery and 20% of adjuvant treatment. We recorded a morbidity of 14% and an early mortality of 7%. Two-year survival was 12.3%. Advanced tumor stage was the only significant prognostic factor (p=0.015). Conclusion: Gastric cancer was the most common digestive tract cancer operated on in the general surgery department of the Ignace Deen national hospital during the study period. Treatment was palliative in the majority of cases. Advanced tumor stage was the only significant factor associated with prognosis.
Abstract: Introduction: gastric cancer remains one of the leading causes of cancer death worldwide. The aim of this work was to study the surgical management and to determine the prognostic factors of gastric cancer in the general surgery department of the Ignace Deen national hospital, Conakry (Guinea). Patients and methods: This was a retrospective descrip...
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High Accuracy Classification of Populations with Breast Cancer: SVM Approach
Philip de Melo,
Mane Davtyan
Issue:
Volume 11, Issue 3, September 2023
Pages:
94-104
Received:
1 July 2023
Accepted:
27 July 2023
Published:
15 August 2023
Abstract: Breast cancer is one of the most common cancers diagnosed in the United States. Breast cancer can occur in both men and women. The number of deaths associated with this disease is steadily declining, largely due to factors such as earlier detection and a new personalized approach to treatment. In this article, we offer a highly accurate and reliable classification approach based on feature engineering and an improved support vector machine (SVM) classifier. We examine a dataset with 30 features and use in-depth data analytics and visualization to pinpoint the top nine features that have a significant impact on classification accuracy. The SVM classification outperformed other classifiers, including kernel extensions, with a high accuracy of 99.12%. The study stresses the value of machine learning in medical diagnosis, notably in the early detection of breast cancer, and indicates the possibility for further research in this area utilizing deep learning architectures. Early detection of breast cancer is critical, and our findings contribute to the growing body of knowledge in this area, opening new avenues for improving cancer diagnosis and patient care.
Abstract: Breast cancer is one of the most common cancers diagnosed in the United States. Breast cancer can occur in both men and women. The number of deaths associated with this disease is steadily declining, largely due to factors such as earlier detection and a new personalized approach to treatment. In this article, we offer a highly accurate and reliabl...
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Ovarian Cancer After Breast Cancer Treatment: Study of 03 Cases in a Guinean Oncological Setting
Bah Malick,
Toure Alhassane Ismael,
Keita Mamady,
Cisse Kalil,
Souare Mamadou Bobo,
Conde Ibrahima Kalil,
Cisse Mohamed Falilou,
Traore Bangaly
Issue:
Volume 11, Issue 3, September 2023
Pages:
105-111
Received:
16 August 2023
Accepted:
1 September 2023
Published:
14 September 2023
Abstract: Introduction: Ovarian cancer, the 1st cause of death from gynecological cancer, is any malignant proliferative process developed in the ovary, which may occur after a treated breast cancer. Objectives: To discuss the management and prognosis of ovarian cancer after breast cancer treatment through a series of 3 clinical cases in a Guinean oncology setting. Case 1: A 60-year-old patient presented with an ulcerating tumor of the uterine cervix, after treatment of a CCI, she benefited from 12 courses of palliative chemotherapy and died 04 months after her last course with decompensated anemia. Case 2: the 75-year-old female patient who presented with abdominal distension with ascites associated with a pelvic mass after treatment of a CCI, died of multivisceral failure. Case 3: A 61-year-old hypertensive diabetic patient presented with abdominal distension and a pelvic-abdominal mass. After treatment of a CCI, she underwent an exploratory laparotomy and died at D15 post-op in hypovolaemic shock. Conclusion: This study shows the need to include monitoring of gynecological organs in patients treated for breast cancer. The diagnosis was late and the prognosis was poor.
Abstract: Introduction: Ovarian cancer, the 1st cause of death from gynecological cancer, is any malignant proliferative process developed in the ovary, which may occur after a treated breast cancer. Objectives: To discuss the management and prognosis of ovarian cancer after breast cancer treatment through a series of 3 clinical cases in a Guinean oncology s...
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Demographic Profile of Patients with Stage-IV Non-Small Cell Lung Cancer: A Single Center Study in Bangladesh
Beauty Saha,
Muhammad Rafiqul Islam,
Nazma Azim,
Salman Bashar Al Aub,
Rashedul Islam,
Ahmed Jabeer
Issue:
Volume 11, Issue 3, September 2023
Pages:
112-115
Received:
22 July 2023
Accepted:
21 August 2023
Published:
20 September 2023
Abstract: Background: In Bangladesh, stage IV non-small-cell lung cancer (NSCLC) is the second most common cancer in males among top five most frequent cancers and the increase in lung cancer risk among women reflects changes during the 20th century. Prior conception on the demographic profile of patients with Stage-IV NSCLC may be helpful for the good management of such patients. Aim of the study: The aim of this study was to form the demographic profile of patients with stage IV non-small-cell lung cancer. Methods: This prospective observational study was conducted in National Institute of Cancer Research & Hospital (NICRH), Dhaka, Bangladesh during the period from September 2019 to August 2020. A total of 108 patients were enrolled purposively. Detailed history taking thorough physical examination was done along with relevant investigations regarding the demographic and clinical status of the participants. Data were collected by semi structured questionnaire and analysis was done with the help of SPSS version 21.0 and MS Office programs as per need. Results: In this study, almost two third (62%) patients were from <60 years’ age group and the rest 38% were from >60 years’ age group. The mean age was found 56.4±12.2 years and the male-female ratio was 4: 1. More than half (52.8%) of the patients came from poor families, 42 (38.9%) were from middle class and the rest 9 (8.3%) were from high class families. More than one third (36.1%) patients were farmer followed by 31 (28.7%) were businessmen, 25 (23.1%) were service holder and the rest 13 (12.1%) were house wife. Conclusion: As per the findings of this study we can conclude that, male may be major prone to stage IV non-small-cell lung cancer. The frequencies of mid-aged patients of NSCLC are alarming.
Abstract: Background: In Bangladesh, stage IV non-small-cell lung cancer (NSCLC) is the second most common cancer in males among top five most frequent cancers and the increase in lung cancer risk among women reflects changes during the 20th century. Prior conception on the demographic profile of patients with Stage-IV NSCLC may be helpful for the good manag...
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Breast Cancer in Women Under 40 Years of Age Surgical Oncology Unit of Donka National Hospital a Retrospective Cohort Study
Malick Bah,
Keita Mamady,
Touré Alhassane Ismael,
Cisse Kalil,
Souare Mamadou Bobo,
Conde Ibrahima Kalil,
Traore Bangaly
Issue:
Volume 11, Issue 3, September 2023
Pages:
116-121
Received:
29 August 2023
Accepted:
13 September 2023
Published:
27 September 2023
Abstract: Objective: To describe the epidemiological, clinical, therapeutic and prognostic aspects of breast cancer in women aged 40 years and younger. Material and methods: This was are retrospective descriptive cohort of 128 months, from April 11, 2007, to December 31, 2017; including patients aged 40 years and younger who had been treated and followed up for breast cancer at the surgical oncology unit of the Donka CHU National Hospital in Conakry. Results: A total of 184 patients were enrolled in this study. The mean age was 33.5 ± 5.4 years. A family history of breast cancer was found in 16 (8.69%) patients. The presence of nodule was the most frequent symptom (91.3%). It was an infiltrating ductal carcinoma in 67.3% of patients. Stages IIIB, IV, and IIIA were the most frequent with 40.2%, 23.3% and 9.2% respectively. Mastectomy was radical in 93.9% of the cases. The associated treatments were chemotherapy (86.4%), radiotherapy (15.7%) and hormone therapy (2.7%). We recorded 79 (42.9%) deaths. Overall survival at 5 years was 31%. Conclusion: Breast cancer in young women remains serious because of its high frequency, more progressive form, and poorer prognosis compared with that of older patients.
Abstract: Objective: To describe the epidemiological, clinical, therapeutic and prognostic aspects of breast cancer in women aged 40 years and younger. Material and methods: This was are retrospective descriptive cohort of 128 months, from April 11, 2007, to December 31, 2017; including patients aged 40 years and younger who had been treated and followed up ...
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